Figure 2.

Nuclear lamin protein levels are down-regulated in HSV-1–infected iDCs, but not mDCs. (a) HFF, iDCs, and mDCs (2 × 10⁶) were mock- or HSV1-RFPVP26–infected (MOI of 2) and harvested 24 hpi. Cells were directly lysed and subjected to immunoblot analyses using specific antibodies raised against phospho-lamin A/C (pLamin A/C), lamin A/C, lamin B1, lamin B2, or ICP0. GAPDH was used to verify equal loading. (b) iDCs and mDCs were mock- or HSV-1–infected or infected with HSV-1 clinical isolates (K4, K7, and K8) and harvested 24 hpi. Samples were analyzed for protein expression of lamin A/C, lamin B1, lamin B2, and ICP0. GAPDH was used as a loading control. Experiments were performed at least three times with cells from different healthy donors and representative data are shown.