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. 2019 Feb 4;218(2):524–540. doi: 10.1083/jcb.201808176

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© 2019 Rosen et al.

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Figure 2. — Bsg25Dgenetically interacts with ens, but not plp. (A) Western blot showing loss of Bsg25D protein in Bsg25D^−/− larval lysates. (B) Extended-focus projections of representative stage 16 hemisegments from indicated genotypes. (C) Bar graph showing mean nuclear spread and SD. For each genotype, n (number of hemisegments) is control, n = 39; Bsg25D^−/−, n = 61; ens^−/+, n = 46; Bsg25D-/Df;ens^−/+, n = 43; Bsg25D;ens^−/+, n = 71; Bsg25D^−/−;BAC, ens^−/+, n = 44. P values were calculated by Student’s t test. (D) Graph showing the percentage of myotubes that have a single nuclear cluster. P values were calculated by Fisher’s exact test from contingency tables comparing ens-/+ to each other genotype. n values (number of myotubes for each genotype) are ens^−/+, 239; Bsg25D^−/−;ens⁻^/+, 296; Bsg25D-/Df;ens⁻^/+, 176; Bsg25D^−/−;BAC, ens^−/+ (rescue), 160. (E) Viability graph showing survival during development for individuals derived from indicated crosses. The following number of individuals were included for each genotype: Bsg25D^−/−×Bsg25D^−/−;ens⁻/TM6, 140; Bsg25D^−/−;ens-/TM6×Bsg25D^−/−, 153; Bsg25D^−/−;ens-/TM6×wt, 140; Bsg25D^−/−;ens^−/+×Bsg25D^−/−, 113; Bsg25D-/Df;ens^−/+×Bsg25D^−/−, 148; Bsg25D^−/−;ens-/TM6×Bsg25D^−/−;BAC[Bsg25D+], 144. F, female; M, male. (F) Extended-focus projections of representative stage 16 hemisegments from indicated genotypes. (G) Bar graph showing mean nuclear spread and SD. For each genotype, number of hemisegments is plp^−/−, n = 47; plp-/Df, n = 24; Bsg25D^−/−; plp^−/−, n = 39; plp-/+, n = 34; Bsg25D^−/−;plp-/+, n = 29. P values were calculated by Student’s t test. The control data in G are the same as in C; for representative image, see first panel in B. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. In all confocal images, red brackets indicate sample nuclear spread measurements. Tropomyosin, green; nuclei, white. Scale bars = 5 µm.