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. 2019 Feb 4;218(2):524–540. doi: 10.1083/jcb.201808176

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© 2019 Rosen et al.

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Figure 5. — Bsg25D overexpression induces nuclear positioning defects in larval myofibers that can be rescued by Ens. (A) Larval motility in larvae overexpressing either GFP (control) or Bsg25D in muscle. For Dmef2-Gal4>UAS-GFP, n = 30 larvae. For Dmef2-Gal4>UAS-Bsg25D, n = 12 larvae. P values were calculated by Student’s t test. (B) Viability graph showing survival during development. n = 100 individuals for each genotype. (C and D) Representative extended focus projections of stained myofibers and nuclear positioning analyses. Green, phalloidin; white, nuclei. Dashed yellow line outlines individual myofibers. Scale bars = 10 µm. Graphs depict mean and SD for three methods for quantifying myonuclear positioning (see Materials and methods). For each genotype, the same images were analyzed with each method. For Dmef2-Gal4>UAS-GFP, n = 40 myofibers. For Dmef2-Gal4>UAS-Bsg25D, n = 45. For Dmef2-Gal4>UAS-GFP;UAS-Bsg25D, n = 23; Dmef2-Gal4>UAS-ens;UAS-Bsg25D, n = 19. Dmef2-Gal4>UAS-GFP data are the same in C and D. P values were calculated by Student’s t test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.