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. 2018 Dec 12;24(1):159–173. doi: 10.1007/s12192-018-0953-7

Fig. 6.

Fig. 6

a X-ray radiographs of Manffl/fl Col2Cre Matn3+/+, Manffl/fl Col2Cre Matn3V194D/V194D and Manffl/fl Col2Cre+ Matn3V194D/V194D mice at 6 weeks, showing the dramatic effect of removal of MANF from cartilage of the mouse model of MED. b Western blot and densitometry measurement of intracellular GRP78 levels in Matn3V194D/V194D primary chondrocytes with and without exogenous MANF (n = 4). c Western blot and densitometry measurement of intracellular matrilin-3 levels in Matn3V194D/V194D primary chondrocytes with and without exogenous MANF (n = 4). d Immunocytochemistry for matrilin-3 showing that exogenous MANF has no effect on the intracellular retention of mutant matrilin-3 (in green) in Matn3V194D/V194D primary chondrocytes. DAPI was used as a counterstain. Scale bars 5 mm (a) and 200 μm (d) (colour figure online)