Fig. 2.

Mitochondrial respiratory function is regulated by Mst1 under hyperglycemia stimulus. a Cellular ATP production was measured in ARPE-19 cells treated with hyperglycemia and/or transfected with Mst1 siRNA. b–c The levels of glucose uptake and lactic acid production were quantified via ELISAs. d–e Mitochondrial membrane potential was assessed using JC-1. The fluorescence intensity of the red-to-green ratio was used to quantify the mitochondrial membrane potential. ARPE-19 cells were treated with hyperglycemia and/or transfected with Mst1 siRNAs. f–i After treatment, proteins were isolated, and the expression levels of mitochondrial respiratory complex components were determined using western blotting. ARPE-19 cells were treated with hyperglycemia and/or transfected with Mst1 siRNAs.*p < 0.05