Skip to main content
. 2018 Dec 1;24(1):125–148. doi: 10.1007/s12192-018-0950-x

Table 2.

The J-proteins from Trypanosoma brucei with their putative T. cruzi, L. major, C. fasciculata, B. saltans, and H. sapiens orthologues

T. bruceia T. cruzicd L. majorc C. fasciculatac B. saltansc H. sapiensc
Type Gene IDb Name Gene IDb Gene IDb Gene IDb Gene IDb Name Localisatione Functionf Reference
I Tb927.2.5160 Tbg972.2.3190 J2 TcCLB.511627.110 Tc_MARK_9726 TCDM_08977 LmjF.27.2400 CFAC1_210029700 CUG85902 DnaJA1 CYT Protein aggregation and refolding Ludewig et al. 2015
Terada et al. 2005
Ahrendt and Braun 2010
Burger et al. 2014
Tb927.10.2290 Tbg972.10.2780 J3 TcCLB.511367.138 Tc_MARK_5591 TCDM_04677 LmjF.21.0490 CFAC1_260011100 CUE68025 – CYT Protein aggregation and refolding Ashburner et al. 2000
Tb927.9.8410 Tbg972.9.4590 J27 TcCLB.510243.30 Tc_MARK_5901 TCDM_05671 LmjF.04.0940 CFAC1_030019200 CUE72942 – MITO Protein aggregation and folding mtDNA maintenance Acestor et al. 2009, 2011
Panigrahi et al. 2009
Niemann et al. 2013
Tb927.11.16740 Tbg972.11.18880 J45 TcCLB.511531.9 Tc_MARK_2746 TCDM_02688 LmjF.32.3300 CFAC1_300054500 CUG88240 – ER MITO ERAD Schlenstedt et al. 1995
Panigrahi et al. 2009
Niemann et al. 2013
Tb927.3.1430 Tbg972.3.1180 J46 TcCLB.509437.40 Tc_MARK_5358 TCDM_10337 LmjF.25.1100 CFAC1_220020000 CUG87582 DnaJB11 ER Protein folding mRNA editing Jin et al. 2009
Tb927.9.12730 Tbg972.9.7790 J50 TcCLB.510743.100 Tc_MARK_4474 TCDM_00202 LmjF.35.2980 CFAC1_300079900 CUG89607 DnaJA3 MITO Protein aggregation and folding mtDNA maintenance Týč et al. 2015
Acestor et al. 2009
Niemann et al. 2013
II Tb927.10.8540 Tbg972.10.10330 J6 TcCLB.506355.50 Tc_MARK_3436 TCDM_11062 LmjF.36.6270 CFAC1_280070900 CUG94252 DnaJB1 CYT Protein (re)folding Freeman and Morimoto 1996
Tb927.11.15130 Tbg972.11.17000 J7 TcCLB.509157.80 Tc_MARK_2851 TCDM_10924 LmjF.32.1940 CFAC1_190031300 CUG94211 – MITO FLAGELLAR ? Acestor et al. 2009
Panigrahi et al. 2009
Niemann et al. 2013
Subota et al. 2014
III Tb927.11.16980 Tbg972.11.19710 J1 TcCLB.511537.50 Tc_MARK_2772 TCDM_02646 LmjF.32.3030 CFAC1_300057600 CUE71254 – CYT, NUC Protein (re)folding Louw et al. 2010b
Tb927.10.5660 Tbg972.10.6880 J5 TcCLB.504163.100 Tc_MARK_8575 TCDM_01101 LmjF.36.1330 CFAC1_250022200 CUG21319 – MITO Protein folding Protein import-MITO Panigrahi et al. 2009
Niemann et al. 2013
Tb927.9.8410 Tbg972.9.4590 J8 TcCLB.510243.30 Tc_MARK_5901 TCDM_05671 LmjF.04.0940 CFAC1_210013600 CUE72942 DnaJC15? MITO, GLYCO Protein aggregation and folding mtDNA maintenance Hatle et al. 2013
Acestor et al. 2009
Niemann et al. 2013
Güther et al. 2014
Tb927.6.3850 Tbg972.6.3630 J9 TcCLB.511517.44 Tc_MARK_2007 TCDM_02176 – CFAC1_140009800 ACI15980 – MITO ? Peikert et al. 2017
Tb927.7.6660 Tbg972.7.760 J10 TcCLB.510055.140 Tc_MARK_7736 TCDM_09211 LmjF.17.0460 CFAC1_090011100 CUG14142 – MITO Acestor et al. 2009
Peikert et al. 2017
Tb927.9.8160 Tbg972.9.4440 J11 TcCLB.510241.70 Tc_MARK_5912 TCDM_05687 LmjF.04.0780 CFAC1_030017200 CUG91719 – MITO Acestor et al. 2009
Niemann et al. 2013
Tb927.4.2970 Tbg972. 4.2970 J12 TcCLB.506435.50 Tc_MARK_2622 TCDM_03557 – CFAC1_230048800 CUG94092 – MITO ? –
Tb927.10.12530 Tbg972.10.12530 J13 TcCLB.509809.20 Tc_MARK_4997 LmjF.18.1490 CFAC1_140024700 CUG91826 DnaJC24? CYT, NUC Dipthamide synthesis Liu et al. 2004
Tb927.5.2880 Tbg972.5.4050 J14 TcCLB.507063.180 LmjF.08.0990 CFAC1_200014300 CUG33795 – CYT, NUC ? –
Tb927.10.14730 Tbg972.10.17890 J15 TcCLB.503833.20 Tc_MARK_4789 TCDM_00497 LmjF.19.0080 CFAC1_170007200 CUG02245 – CYT ? Nett et al. 2009
Tb927.1.1960 Tbg972.1.1130 J16 TcCLB.506473.20 Tc_MARK_6047 TCDM_00922 LmjF.20.1130 CFAC1_180019100 CUI12704 DnaJC2 CYT Translation Otto et al. 2005
Nett et al. 2009
Tb927.1.4300 Tbg972.1.2850 J17 TcCLB.506529.260 Tc_MARK_5675 TCDM_05005 LmjF.12.1110 CFAC1_010014800 CUG89438 – CYT, NUC ? –
Tb927.11.1010 Tbg972.11.1070 J18 TcCLB.506925.470 Tc_MARK_6358 TCDM_01954 LmjF.27.0410 CFAC1_230048800 CUG94092 – MITO Protein import-MITO Ashburner et al. 2000
Acestor et al. 2009
Peikert et al. 2017
Tb927.4.650 Tbg972.4.400 J19 TcCLB.507053.120 Tc_MARK_2469 LmjF.34.4080 CFAC1_290072000 CUI15604 – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Peikert et al. 2017
Tb927.10.5040 Tbg972.10.6100 J20 TcCLB.510293.50 Tc_MARK_8503 TCDM_01182 LmjF.36.0610 CFAC1_250013400 CUG62628 – MEM Protein import Ashburner et al. 2000
Tb927.7.540 Tbg972.7.420 J21 TcCLB.506287.90 Tc_MARK_965 TCDM_8503 LmjF.26.1410 CFAC1_290023200 CUI15434 – MITO, GLYCO Protein import Panigrahi et al. 2009
Güther et al. 2014
Peikert et al. 2017
Niemann et al. 2013
Tb927.10.6610 Tbg972.10.8080 J22 TcCLB.510187.330 Tc_MARK_3742 TCDM_06560 LmjF.36.2110 CFAC1_250032000 CUG93498 – MITO, GLYCO ? Niemann et al. 2013
Zíková et al. 2017
Güther et al. 2014
Tb927.10.13830 Tbg972.10.17000 J23 TcCLB.507993.30 Tc_MARK_4871 LmjF.18.0330 CFAC1_140009800 ACI15980 – MITO Protein import-MITO Ashburner et al. 2000
Acestor et al. 2009
Niemann et al. 2013
Tb927.6.3120 Tbg972.6.2910 J24 TcCLB.511751.60 Tc_MARK_1932 LmjF.30.1790 CFAC1_260039300 CUG86810 – CYT, NUC ? –
Tb927.7.680 Tbg972.7.590 J25 TcCLB.506289.74 Tc_MARK_979 TCDM_07562 LmjF.26.1270 CFAC1_290021600 CUG93121 – MITO ? Panigrahi et al. 2009
Niemann et al. 2013
Tb927.7.6200 Tbg972.7.7170 J26 TcCLB.506513.30 Tc_MARK_7763 LmjF.17.0040 CFAC1_090006000 CUG74938 – MITO, GLYCO Protein import-MITO Ashburner et al. 2000
Acestor et al. 2009
Güther et al. 2014
Niemann et al. 2013
Tb927.7.740 Tbg972.7.670 J28 TcCLB.506135.40 TCDM_01252 LmjF.26.1200 CFAC1_290020200 CUG93098 – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Peikert et al. 2017
Tb927.11.5710 Tbg972.11.6430 J29 TcCLB.506203.50 Tc_MARK_311 TCDM_00777 LmjF.24.1080 CFAC1_210020200 CUF82364 – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Tb927.8.1010 Tbg972.8.590 J30 TcCLB.508569.120 Tc_MARK_8237 TCDM_01742 LmjF.07.0780 CFAC1_080017400 CUI14395 – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Peikert et al. 2017
Tb927.3.2290 Tbg972.3.2270 J32 TcCLB.508479.280 Tc_MARK_5111 TCDM_08321 LmjF.25.2190 CFAC1_230010600 CUG43629 – ER ? Field et al. 2010
Tb927.10.9840 Tbg972.10.12010 J33 TcCLB.504147.60 Tc_MARK_2256 TCDM_02891 LmjF.36.4470 CFAC1_280050000 CUG72683 – CYT ? –
Tb927.9.10010 Tbg972.9.5780 J34 TcCLB.506887.90 Tc_MARK_4329 TCDM_00009 LmjF.35.4630 CFAC1_300097800 CUG92668 – ER, MITO Goldshmidt et al. 2008
Acestor et al. 2009
Panigrahi et al. 2009
Peikert et al. 2017
Niemann et al. 2013
Tb927.7.4590 Tbg972.7.5200 J35 TcCLB.506605.150 Tc_MARK_7879 TCDM_05890 LmjF.14.0110 CFAC1_110006300 CUF84499 – MITO ? Panigrahi et al. 2009
Tb927.3.1760 Tbg972.3.1610 J36 TcCLB.510091.50 Tc_MARK_5057 TCDM_03945 LmjF.25.1690 CFAC1_230016200 – DnaJC20 MITO FeS cluster biogenesis Mokranjac et al. 2003
Uhrigshardt et al. 2010
Acestor et al. 2009
Tb927.10.12640 Tbg972.10.15230 J37 TcCLB.503455.10 Tc_MARK_4988 Tc_MARK_4989 TCDM_05256 LmjF.18.1430 – CUG91818 – CYT, NUC ? –
Tb927.6.3730 Tbg972.6.3500 J38 TcCLB.506941.270 LmjF.30.2450 CFAC1_140023800 CUF23573 – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Peikert et al. 2017
Tb927.9.14180 Tbg972.9.8890 J39 TcCLB.510759.134 Tc_MARK_4558 TCDM_03405 – CFAC1_250032000 – – MITO ? Niemann et al. 2013
Tb927.8.4470 Tbg972.8.4230 J40 TcCLB.508989.60 LmjF.10.1050 CFAC1_040019100 CUG90885 – MITO ? Peikert et al. 2017
Tb927.4.3980 Tbg972.4.4110 J41 – – CFAC1_270012300 CUG89969 – CYT, GOLGI Intracellular trafficking Ashburner et al. 2000
Tb927.10.12380 Tbg972.10.14920 J42 TcCLB.507625.110 Tc_MARK_5009 TCDM_09879 LmjF.18.1650 CFAC1_140028800 – – CYT Protein folding Ashburner et al. 2000
Tb927.9.10950 Tbg972.9.6470 J43 TcCLB.508461.240 Tc_MARK_4379 TCDM_00080 LmjF.35.4040 CFAC1_300054500 – – CYT ? –
Tb927.8.7010 Tbg972.8.7230 J44 TcCLB.509911.100 Tc_MARK_1523 LmjF.31.3100 CFAC1_270068300 CUE89851 – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Peikert et al. 2017
Tb927.8.1030 Tbg972.8.610 J48 TcCLB.508569.140 Tc_MARK_8239 TCDM_01739 LmjF.31.3100 – – – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Peikert et al. 2017
Tb927.6.2480 Tbg972.8.2240 J49 TcCLB.509965.229 TCDM_01497 LmjF.30.1030 CFAC1_260030200 CUG89958 – MITO Protein import-MITO Ashburner et al. 2000
Panigrahi et al. 2009
Peikert et al. 2017
Tb927.4.2220 Tbg972.4.2160 J51 TcCLB.506559.430 Tc_MARK_452 TCDM_04700 LmjF.34.2430 CFAC1_290053200 CUG93032 – CYT Protein folding Ashburner et al. 2000
Tb927. 10.4900 Tbg972.10.5950 J52 TcCLB.504203.60 Tc_MARK_8493 TCDM_01195 LmjF.36.0500 CFAC1_250012000 CUG91204 DnaJC7 CYT Protein folding quality control Brychzy et al. 2003
Tb927.7.3630 Tbg972.7.4040 J53 TcCLB.510407.80 Tc_MARK_8808 TCDM_06728 LmjF.14.1330 CFAC1_110026000 CUG92395 DnaJC3 ER, MITO, GLYCO ER protein synthesis Yan et al. 2002
Goodman et al. 2011
Güther et al. 2014
Tb927.11.8420 Tbg972.11.9590 J55 TcCLB.506931.30 LmjF.28.1270 CFAC1_300022100 CUF97845 – SEC ? –
Tb927.2.3960 Tbg972.2.2180 J56 TcCLB.511109.90 Tc_MARK_9638 TCDM_14441 LmjF.33.2690 CFAC1_210037700 – DnaJB3? NUC Protein folding Ashburner et al. 2000
Tb927.11.5920 Tbg972.11.6680 J58 TcCLB.508515.120 Tc_MARK_6411 TCDM_00738 LmjF.24.1300 CFAC1_210023600 CUG89365 DnaJC14 ER, MITO, CYT Cell surface export Jung et al. 2016
Tb927.6.3500 Tbg972.6.3250 J59 TcCLB.506941.9 Tc_MARK_ 1973 TCDM_06339 TCDM_06340 LmjF.30.2210 CFAC1_260045500 CUF24956 DnaJC13 CYT Endosome trafficking Girard et al. 2005
Girard and McPherson 2008
Tb927.11.13600 Tbg972.11.15180 J60 TcCLB.506753.160 Tc_MARK_5565 TCDM_05717 LmjF.08.0650 CFAC1_050025800 CUG87832 – MITO ? Panigrahi et al. 2009
Tb927.10.2460 Tbg972.10.3050 J62 TcCLB.412943.9 Tc_MARK_3379 TCDM_04012 TCDM_04013 LmjF.34.0040 CFAC1_290027800 CUF08277 – MEM Protein import Ashburner et al. 2000
Tb927.11.13830 Tbg972.11.15450 J63 TcCLB.509161.110 Tc_MARK_2986 TCDM_09129 LmjF.32.0590 CFAC1_190012200 CUI15501 – MITO Acestor et al. 2009
Tb927.4.880 Tbg972.4.680 J65 TcCLB.508257.160 Tc_MARK_2488 TCDM_02481 LmjF.34.3870 CFAC1_290069300 CUI15574 – MITO Protein folding Ashburner et al. 2000
Tb927.10.5180 Tbg972.10.6290 J67 TcCLB.510297.30 TCDM_01165 LmjF.36.0760 CFAC1_250015000 CUG94364 – MITO Protein folding Ashburner et al. 2000
Tb927.11.10950 Tbg972.11.12260 J69 TcCLB.509823.10 Tc_MARK_7219 TCDM_09821 LmjF.36.4970 CFAC1_280056000 CUG89391 – MITO ? Panigrahi et al. 2009
Tb927. 8.8310 Tbg972. 8.8650 J70 – – – – – GLYCO ? –
Tb927. 9.12530 Tbg972.9.7690 J71 TcCLB.510741.190 Tc_MARK_4463 TCDM_00188 LmjF.35.3090 CFAC1_300081200 CUG89593 – MITO Protein import-MITO Ashburner et al. 2000
Tb927.11.9060 Tbg972.11.10120 J72 TcCLB.507077.30 Tc_MARK_1361 TCDM_07079 – – – – CYT ? –
Tb927.9.1560 Tbg972.9.280 J73 TcCLB.510347.50 Tc_MARK_2404 TCDM_08512 LmjF.26.2520 CFAC1_160029000 CUG90840 – NUC Goos et al. 2017
IV Tb927.7.990 Tbg972.7.940 J31 TcCLB.506729.50 Tc_MARK_1042 TCDM_01293 LmjF.26.0940 CFAC1_290017000 – – MITO ? Acestor et al. 2009
Panigrahi et al. 2009
Niemann et al. 2013
Tb927.1.1230 Tbg972.1.570 J47 TcCLB.511423.170 Tc_MARK_6003 TCDM_01017 LmjF.20.0550 CFAC1_180012900 CUG67522 – MITO Protein folding Panigrahi et al. 2009
Tb927.8.6310 Tbg972.8.6370 J68 TcCLB.503885.70 LmjF.24.1910 CFAC1_250049500 CUG31915 DnaJC19 MITO Protein import-MITO Ashburner et al. 2000

CYT cytosol, MITO mitochondria, NUC nucleus, ER endoplasmic reticulum, MEM plasma membrane/membrane bound, GOLGI Golgi apparatus, GYLCO glycosomes, SEC secreted

aThe nomenclature for the J-proteins of T. b. brucei and T. b. gambiense were derived from Folgueira and Requena (2007)

bGene IDs for the J-proteins from T. b. brucei, T. b. gambiense, T. cruzi, L. major and C. fasciculata were obtained from TriTrypDB database (http://tritrypdb.org/tritrypdb/; Aslett et al. 2010). The gene IDs for the J-proteins from B. saltans were retrieved from NCBI (https://www.ncbi.nlm.nih.gov/)

cOrthologues identified from T. cruzi, L. major, C. fasciculata, and Homo sapiens were determined by reciprocal BLASTP analysis

dThe gene IDs for orthologues identified in three strains of T. cruzi are listed. T. cruzi CL Brener Esmeraldo-like (TcCLB), T. cruzi Dm28c (TCDM), and T. cruzi marinkelli strain B7 (Tc_MARK)

eSubcellular localizations for the T. b. brucei and T. b. gambiense J-proteins were either predicted using the online prediction servers and/or determined using various proteomic datasets listed in the materials and methods

fThe predicted cellular role and functions for each J-protein from T. brucei were implied from either Gene Ontology (Ashburner et al. 2000), or published literature on the identified functions/cellular roles of their identified orthologues