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. 2019 Jan 30;9:10. doi: 10.3389/fcimb.2019.00010

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Copyright © 2019 Luo, Tan, Yu, Lin, Hou, Mao, Liu, Wang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the optimal number of PFMCs for performing PF T-SPOT. (A) Representative pictures showing inaccurate PF T-SPOT results If 2.5 × 10⁵ PFMCs are added to T-SPOT well (patient 1: PHA SFCs are inaccurate; patient 2 to 5: Mtb-specific antigens and PHA SFCs are inaccurate; patient 6 and 7: negative well, Mtb-specific antigens, and PHA SFCs are inaccurate). (B) The PF T-SPOT results under different number of PFMCs. Different number of PFMCs (1 × 10⁴, 5 × 10⁴, 1 × 10⁵, 1.5 × 10⁵, 2.5 × 10⁵) of one representative patient was added to T-SPOT well. The number in the upper left corner of each graph indicates the number of SFC in each well of PF T-SPOT assay. ESAT-6, early secreted antigenic target 6; CFP-10, culture filtrate protein 10; PHA, phytohaemagglutinin.