Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 30;10:85. doi: 10.3389/fmicb.2019.00085

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Jin, Zhang, Zhu, Peng, Weng, Zhao, Liu, Bai, Cheng, Jin and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Plasmid mediated expression of exsA restored T3SS in the ΔnrtR mutant. (A) The relative exsA mRNA levels in PAK, the ΔnrtR mutant and ΔnrtR/att7:: nrtR strain. Total RNA was isolated under T3SS inducing and non-inducing conditions and the exsA mRNA levels were determined by real-time PCR using rpsL as the internal control. ^∗P < 0.05, ^∗∗P < 0.01, by Student’s t-test. (B) Bacteria harboring an exsA-Flag driven by a lac promoter or the empty vector pDN19 were grown to an OD₆₀₀ of 1.0 in LB with or without EGTA. Proteins from equivalent number of bacterial cells of indicated strains were separated on SDS-PAGE and probed with an anti-ExoS antibody or an anti-RNA polymerase beta subunit antibody. Expression levels of the ExsA-Flag were determined with an anti-FLAG antibody (C).