Table 2.
Primers used in this study.
| Primer | Sequence 5′–3′b | Source |
|---|---|---|
| PA4916UFa | CTCGGAATTCTTCCAGACGAAGAAGTCGTAG | This study |
| PA4916UR | CTGCTCTAGACGTCACTCCTCTCTTCAGCCC | This study |
| PA4916DF | GAGCTCTAGACCTGCCGCGCTTGCTAGACG | This study |
| PA4916DR | GCCCAAGCTTAAATCATCGAGTCGCTGGTCCCC | This study |
| nadD2UF | GGAATTCGAAGACCTCCACCTCCAGTGTCG | This study |
| nadD2UR | GCTCTAGAAGAGAGGAGTGACGATGAGTTCAG | This study |
| nadD2DF | GCTCTAGAAGAAAATACCTTCCACTGCG | This study |
| nadD2DR | CCCAAGCTTGAGCAGGTTCTGCACAATGC | This study |
| cyaAUF | GGAATTCCGGCATCCGTTGTTCCGCGCGGAGATCCAG | This study |
| cyaAUR | GCTCTAGAGGGCGTCCGGGCACAGGCAAGGCCAGGCG | This study |
| cyaADF | GCTCTAGACCCAGCGCCGCACCGCGCGGGGCTCGAC | This study |
| cyaADR | CCCAAGCTTCGCCGGCGAAGGCAAGGTCTCGATCCTC | This study |
| cyaBUF | GGAATTCGGAAAGTCAGGTCGGACGCTTCCGCGATG | This study |
| cyaBUR | GCTCTAGAGCGCTGGAGAGGATCCCTGTGTATTTTCG | This study |
| cyaBDF | GCTCTAGAGTTCGTCGAACGCCGCCGGCAGTTCGTCGCGCC | This study |
| cyaBDR | CCCAAGCTTCCGCTCGGCTGGGCCGCGCGGCGCTGGC | This study |
| vfrUF | CTCGGAATTCGTAGCAGATGTCGTAGATGTTG | This study |
| vfrUR | CTGGGGTACCCGAGTCCCGAAAGAATAAAG | This study |
| vfrDF | GAGGGGTACCTGGTGCATGTGAAAGGAAAGAC | This study |
| vfrDR | GCCCAAGCTTGCGACCAGCCTGCACGAG | This study |
| PA4916PF | GCGAGCTCTCCTTGCTGCCCAGGCGCAGC | This study |
| PA4916PR | AACTGCAGTCACTTGCCGAAGGCGTGGCGGTGG | This study |
| PA4916F | AACTGCAGGGCGGTCTGAAGAGAGGAGTGACG | This study |
| PA4916R | CCCAAGCTTTGCCGCACCCGTTTGTCAGG | This study |
| cyaAFown | GGGGTACCGCAGCGCATCCTCGCCAGCGGCGAG | This study |
| cyaAFtac | GGGGTACCCTGGCCTTGCCTGTGCCCGGACGCCC | This study |
| cyaAR | CCCAAGCTTTCACTTGTCGTCATCGTCCTTGTAGTCTTGTTCCAGCAGCGCCTGGTTCAGCGCCG | This study |
| cyaBFown | GGGGTACCTCGCCGAGTTCTACCCCTACTACCTGCAG | This study |
| cyaBFtac | GGGGTACCATACACAGGGATCCTCTCCAGCGCATG | This study |
| cyaBR | CCCAAGCTTTCACTTGTCGTCATCGTCCTTGTAGTCGAGGATGACCTTGTCGCGCAGGCGTTCGG | This study |
| nadD2OF | GGAATTCCAAGCACTTGTACTACAAAATTTCGCAG | This study |
| nadD2ORHis | CCCAAGCTTTCAATGATGATGATGATGATG GCCCGCCTGGCGTTCGCCGCCATAGCAGTG | This study |
| nadD2ORFlag | CCCAAGCTTTCACTTGTCGTCATCGTCCTTGTAGTC GCCCGCCTGGCGTTCGCCGCCATAGCAGTG | This study |
| PA4918-20OF | GCTCTAGACAAGCGGAGGCTTCCATGAATCGCCCCAGC | This study |
| PA4918-20OR | CCCAAGCTTTCAGGGCGCCTTCGGCAGTTCGCGCTTGTG | This study |
| cpdAFown | GGGGTACCCGCAGGCCTCGCGCCGGGTCGCGCTGAGCG | This study |
| cpdAR | CCCAAGCTTTCACTTGTCGTCATCGTCCTTGTAGTCGTATCCGGCGGTGTCGTAGTCCACTTC | This study |
| pET16b-nadD2F | TTTTCTCCATGGGCCGTGATGAAATAAGTTCCCGGATTCGCCGA | This study |
| pET16b-nadD2R | CCGCTCGAGGCCCGCCTGGCGTTCGCCGCCATAGCAG | This study |
| pET28a-cyaB217-463F | CATGCCATGGGCAAGAGCGTGCGCCTGGAAACCCAGC | This study |
| pET28a-cyaB217-463R | CCGCTCGAGGAGGATGACCTTGTCGCGCAGGCGTTCGG | This study |
| pMMB67EH-cyaB217-463F | GGGGTACCAAGAGCGTGCGCCTGGAAACCCAGC | This study |
| pMMB67EH-cyaB217-463R | CCCAAGCTTTCAATGATGATGATGATGATG GAGGATGACCTTGTCGCGCAGGCGTTCGG | This study |
| pMMB67EH-cyaA-hisR | CCCAAGCTTTCAATGATGATGATGATGATG TTGTTCCAGCAGCGCCTGGTTCAGCGCCG | This study |
| cyaB-SDF | AATTCATACACAGGGATCCTCTCCAGCGCATGGGTAC | This study |
| cyaB-SDR | CCATGCGCTGGAGAGGATCCCTGTGTATG | This study |
| lacP1F | GAATTCGCCCAATACGCAAACCGC | This study |
| lacP1R | GGATCCTCAGGCGAAAGGGGGATGTGCTG | This study |
| pTn7L | ATTAGCTTACGACGCTACACCC | This study |
| pglmS-up | CTGTGCGACTGCTGGAGCTGA | This study |
| qPCR primer | ||
| qcyaAF | CTTCAAGGAGCAGGTATTC | This study |
| qcyaAR | TTCGAGATGGCGATAGAC | This study |
| qcyaBF | GACCTGCTCAACAACTACC | This study |
| qcyaBR | GACGAACTTGTCGATGGT | This study |
| qcpdAF | GCGGATCGACCTGATTCTC | This study |
| qcpdAR | CTGCGGAAGCGTGTGTAG | This study |
| qPA4918F | GTCATCGAATACCTGAGG | This study |
| qPA4918R | GTTCTTCACGCAGTAGTC | This study |
| qnadD2F | GGTGTATTGCGAACCGATC | This study |
| qnadD2R | GCTTCCAGCAGGTCGATG | This study |
| qexsAF | CACGTCGGATAATCCTGATT | This study |
| qexsAR | TAGCGGAGAGGCATGAATA | This study |
| qprpLF | TATCGTATTTCGCCGACTCCC | This study |
| qprpLR | GCGAGTTGCCGTTGTTCAG | This study |
| qtoxAF | CGAGATGGGCGACGAGTTG | This study |
| qtoxAR | TGATGACCGTGGGCTTGATGT | This study |
| qrpsLF | CAAGCGCATGGTCGACAAGAG | This study |
| qrpsLR | ACCTTACGCAGTGCCGAGTTC | This study |
aF, forward; R, reverse; U, upstream of specific gene; D, downstream of specific gene; P, promoter of specific gene; own, native promoter of specific gene; tac, tac promoter; O, for over-expression plasmid construction; SD, Shine-Dalgarno sequence. bThe underlines are the sites of restriction enzymes.