Figure 3.

Hair follicle marker SOX2 is negative in apocrine sweat glands in prenatal sheepskin as detected by immunohistochemistry. SOX2 is specifically localized in the dermal condensates/dermal papilla of primary wool follicles, not in those of secondary wool follicles and apocrine sweat glands. (A–E) The strong positive signals of SOX2 antibody staining are restricted in the dermal condensates associated with the primary wool follicle placodes (A–D) in early stages and in the dermal papilla in later stage (E). The secondary wool follicles and apocrine sweat glands are all negative for SOX2 immunohistochemistry. (F) The negative control is displayed without applying the primary antibody in immunohistochemistry. A–D Bar, 50 μm. E and F Bar, 100 μm.