Figure 4.

The localization of PDGFRB by immunohistochemistry is positive in dermal-originated cells and negative in apocrine sweat glands in the prenatal sheepskin. (A) PDGFRB is highly expressed in the dermal condensates of primary wool follicles and occasionally in dermal fibroblast before apocrine sweat gland induction; (B–D) PDGFRB is expressed in dermal condensates of primary and secondary wool follicles, and not in the precursor cells or the bud of apocrine sweat glands; (E) Strong PDGFRB expression is detected in the upper dermis of the sheepskin, especially the location surrounding the wool follicles. PDGFRB is surprisingly negative in the dermal papilla of the well-developed wool follicles and also negative in the apocrine sweat glands. (F) The negative control is displayed without applying the primary antibody in immunohistochemistry. A–D Bar, 50 μm. E and F Bar, 100 μm.