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. 2019 Jan 30;10:95. doi: 10.3389/fimmu.2019.00095

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Copyright © 2019 Rip, de Bruijn, Appelman, Pal Singh, Hendriks and Corneth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BCR and TLR9 stimulation have different effects on CD19-hBtk B cells. Proportions of B cells in apoptotic or cycling fractions, as determined by propidium iodide (PI) DNA content staining of in vitro cultured purified naïve splenic B cells that were stimulated with 10 μg αIgM (A), 2 μM CpG (B), or αIgM and CpG combined (C), with or without ibrutinib (Ibr, 1 μM) as indicated (left). Symbols represent individual mice and bars indicate mean values. Representative flow cytometry graphs of WT and CD19-hBtk B cells are shown on the right, with the gating applied for apoptotic cells (sub-G1), resting cells (G0/G1), and dividing (S/G2/M) B cells. CD19-hBtk and WT mice were 8–10 weeks old; n = 4–5 per group; ^*p < 0.05, ^**p < 0.01 by Mann–Whitney U-test.