Figure 3.

CD19-hBtk B cells show increased upregulation of activation markers upon synergistic BCR and TLR9 stimulation. Proportions of B cells positive for CD86 (A), CD25 (B), CD80 (C), and CD69 (D) upon 72 h of stimulation of MACS-purified naïve splenic B cells with 10 μg αIgM (αIgM) and/or 2 μM CpG, with or without ibrutinib (Ibr, 1 μM), as indicated (left). Symbols represent individual mice and bars indicate mean values. Representative histogram overlays are depicted on the right. CD19-hBtk and WT mice were 8–10 weeks old; n = 4–5 per group; ^*p < 0.05, ^**p < 0.01 by Mann–Whitney U-test.