Figure 3.

Isolation and screening for L-Asparaginase producing micro-organism with different nitrogen source, on plate supplemented with BTB dye. ‘a’ indicates the Isolate grown on plate containing NaNO₃ as sole nitrogen source. ‘b’ indicates the Isolate grown on plate containing urea as a sole nitrogen source. ‘c’ indicates the Isolate grown on plate containing glutamine as a sole nitrogen source. ‘d’ indicates the Isolate grown on plate containing L-Asparagine as a sole nitrogen source. Table 3 contains the list of the samples. Numbers 1–29 are isolated that produced L-Asparaginase free of glutaminase and urease, 35, 38 and 39 produced L-Asparaginase and glutaminase free of Urease, and 44 produced L-Asparaginase and urease free of glutaminase. The remaining isolated did not produce any of the enzymes.