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. 2019 Feb 5;9:1423. doi: 10.1038/s41598-018-38094-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Microscopic images of five isolates that exhibited the highest zone indices and produced L-Asparaginase free of glutaminase and urease [taken using CETI Max II Binocular Microscopes 1202.4000 M]. Column 1: 40x magnification, Column 2: 100x magnification. (b) the phylogenetic tree of Trichosporon asahii IBBLA1, the microbe with highest enzyme activity, based on 5.8SrRNA for strain S1–3.