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. 2019 Feb 5;9:1415. doi: 10.1038/s41598-018-38391-9

Figure 2.

Figure 2

The Qizhu decoction extracts inhibit the migration of primary cancer hepatocytes in DEN-induced HCC. Mice were intragastrically administered DEN (50 mg/kg i.p.) daily for 28 weeks, and primary cancer hepatocytes were then isolated for further experiments. (A) Wound-healing assays with 10 μg/mL QC and QS. The migration of the cells to the wound was visualized at 0, 12, and 24 h with a Leica inverted phase-contrast microscope (×200 magnification); #P < 0.001 vs. the negative control. (B) The levels of E-cadherin, N-cadherin, and GAPDH proteins in primary cancer hepatocytes were analyzed by western blot analysis (B-1), and a corresponding semiquantitative analysis based on optical density was performed using ImageJ software (B-2). #P < 0.001 vs. the control.