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. 2019 Feb 5;9:1415. doi: 10.1038/s41598-018-38391-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effect of Qizhu decoction extracts on HBV-induced hepatitis in mice involves the suppression of NF-κB. (A) The effect of QC on the mRNA expression of TNF-α, IL-1β, and COX-2 in mouse livers was analyzed by real-time PCR. GAPDH was used as an internal control. Each value indicates the mean ± SD and is representative of results from three independent experiments; ^#P < 0.001 vs. the control. (B) The effect of QS on the mRNA expression of TNF-α, IL-1β, and COX-2 in mouse livers was analyzed by real-time PCR. GAPDH was used as an internal control. Each value indicates the mean ± SD and is representative of results from three independent experiments; ^#P < 0.001 vs. the control. (C,D) NF-κB activity in mouse livers was analyzed by western blot (C), and a corresponding semiquantitative analysis based on optical density was performed using ImageJ software (D). The values are presented as the means ± SDs of data from three separate experiments; ^#P < 0.001 vs. the control.