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. 2019 Feb 5;9:1452. doi: 10.1038/s41598-018-36529-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression analyses of PtrCSLD genes in P. trichocarpa. Absolute transcript abundance of PtrCslD1 (A), PtrCslD2 (B), PtrCslD3 (C), PtrCslD4 (D), PtrCslD5 (E), PtrCslD6 (F), PtrCslD7 (G), PtrCslD8 (H), PtCslD9 (I), and PtrCslD10 (J), were examined in young roots (root 1), mature roots (root 2), stems of internodes 1–3 (stem 1–3), leaves, xylem, phloem, and shoot apex. The plasmid containing the gene was used as a standard for establishing a quantitative correlation between the copy number of the target gene transcript molecules and the C_T values. Error bars represent standard errors of triplicate assay.