Table 2.
Strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristicsa | Source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | supE44 lacU169 (Ø80lacZΔM15) hsdR17 (rk-mk-), recA1 endA1 gyrA96 thi-1 relA1 | Woodcock et al., 1989 |
| CC118λpir | araD Δ(ara, leu) ΔlacZ74 phoA20 galK thi-1rspE rpoB argE recA1 λpir | Herrero et al., 1990 |
| HB101 | F- Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (SmR) xyl-5 mtl-1 recA13 thi-1 | Boyer and Roulland-Dussoix, 1969 |
| BL21 (DE3) | F- ompT gal dcm lon hsdSB (rB-mB-) λ(DE3) | Jeong et al., 2009 |
| Pseudomonas putida | ||
| KT2440 | Wild type; Derivative of P. putida mt-2, cured of pWWO | Belda et al., 2016 |
| KT2440R | RifR derivative of P. putida KT2440; wild type | Espinosa-Urgel and Ramos, 2004 |
| KT2440RTn7-Sm | Extragenic site-specific insertion of miniTn7; RifR, SmR | Matilla et al., 2007 |
| KT2440R_CheA1 | pp1492::mini-tn5-Km; RifR, KmR | Duque et al., 2007 |
| KT2440R_CheA2 | Δpp4338::km; constructed by marker exchange, RifR,KmR | This study |
| KT2440R_CheA3 | pp4988::mini-tn5-Km; RifR, KmR | Duque et al., 2007 |
| Plasmids | ||
| pET28b(+) | KmR; Protein expression plasmid | Novagen |
| pRK600 | CmR; mob tra | Finan et al., 1986 |
| pUC18Not | ApR; identical to pUC18 but with two NotI sites flanking pUC18 polylinker | Herrero et al., 1990 |
| p34S-Km3 | KmR, ApR; Km3 antibiotic cassette | Dennis and Zylstra, 1998 |
| pKNG101 | SmR; oriR6K mob sacBR | Kaniga et al., 1991 |
| pCdrA::gfpC | ApR, GmR; FleQ dependent c-di-GMP biosensor | Rybtke et al., 2012 |
| pBBR1MCS-5 | GmR; oriRK2 mobRK2 | Kovach et al., 1995 |
| pPP4888 | KmR; pET28b(+) derivative containing DNA fragment encoding PP4888-LBD cloned into NdeI and BamHI sites | This study |
| pMAMV287 | ApR; 2.2-kb BamHI/HindIII PCR product containing a fragment of pp4338 was inserted into the same sites of pUC18Not | This study |
| pMAMV289 | ApR, KmR; a 0.8-kb PstI fragment internal to pp4338 of pMAMV287 was replaced for a 1-kb PstI fragment containing km3 cassette of p34S-Km3 | This study |
| pMAMV290 | SmR, KmR; 2.4-kb NotI fragment of pMAMV289 was cloned at the same site in pKNG101 | This study |
aAp, ampicillin; Gm, gentamycin; Km, kanamycin; Rif, rifampin; Sm, streptomycin.