Table 3.
Oligonucleotides used in this study.
| Name | Sequence (5′–3′) | Description/purpose |
|---|---|---|
| PP4338-BamHI-F | TAATGGATCCAAGCGCGTCACCACACTG | Forward primer for mutagenesis of PP_4338 |
| PP4338-HindIII-R | TAATAAGCTTGTACGCGACAGGTCGAGGTG | Reverse primer for mutagenesis of PP_4338 |
| PP4888LBD-NdeI-F | AACATATGACCCGAAGCACGGTCACCGCC | Forward primer to clone the region encoding PP4888-LBD into pET28b(+) |
| PP4888LBD-BamHI-R | AAGGATCCCTAGCGCAGCTCGGCAGCCGCAT | Reverse primer to clone the region encoding PP4888-LBD into pET28b(+) |
| qPP_McpR_F | CATACTGGTTGGCGGCTTTT | RT-qPCR of PP_0317 (mcpR) |
| qPP_McpR_R | TCCGAGCAGATCAACAAGGT | RT-qPCR of PP_0317 (mcpR) |
| qPP_0320_F | ACCGGCCATTACTACAACGA | RT-qPCR of PP_0320 (mcpH) |
| qPP_0320_R | ATGTTGATGAACCGTTCGGC | RT-qPCR of PP_0320 (mcpH) |
| qPP_CtpL_F | TGCTGGTGACCGTGTGTATC | RT-qPCR of PP_0562 (ctpL_PP) |
| qPP_CtpL_R | CCCAGATAGCGCTCCATCAG | RT-qPCR of PP_0562 (ctpL_PP) |
| qPP_McpU_F | ATTGGCCTGTACCTGGTGTT | RT-qPCR of PP_1228 (mcpU) |
| qPP_McpU_R | GGGACCAGTACAGCGAGAAA | RT-qPCR of PP_1228 (mcpU) |
| qPP_McpG_F | CTTCCTCACGGTCTACCTGG | RT-qPCR of PP_1371 (mcpG) |
| qPP_McpG_R | GTTCATGCCGTCCTTGTACC | RT-qPCR of PP_1371 (mcpG) |
| qPP_1488_F | TGCTGATCAGGCATCCAGTC | RT-qPCR of PP_1488 (wspA_PP) |
| qPP_1488_R | AAGCTTGGCATTGACCAGGT | RT-qPCR of PP_1488 (wspA_PP) |
| qPP_2111_F | GAGATCAGCCGCAACATCAG | RT-qPCR of PP_2111 (aer2) |
| qPP_2111_R | GTCAGTTCTTCGCTCAGCAG | RT-qPCR of PP_2111 (aer2) |
| qPP_CtpH_F | ATGCAGCAGACCATCGACAT | RT-qPCR of PP_2120 (ctpH_PP) |
| qPP_CtpH_R | TTTGCCTATCCGTGTGCTGT | RT-qPCR of PP_2120 (ctpH_PP) |
| qPP_McpA I2_F | AGCAGACCAACCTGCTGGCACTGAAC | RT-qPCR of PP_2249 (mcpA) |
| qPP_McpA_R5 | TGCCATCGATTTCGCCAATG | RT-qPCR of PP_2249 (mcpA) |
| qPP_2257_F4 | ACTGCAATGAACCAGATGGC | RT-qPCR of PP_2257 (aer1) |
| qPP_2257_R3 | CAGGTTGGTTTGCTCGGC | RT-qPCR of PP_2257 (aer1) |
| qPP_2643_F | GGAGCTGAACAACAAGAGCC | RT-qPCR of PP_2643 (pcaY_PP) |
| qPP_2643_R | CGTATTCGGCAAAGGTAGCC | RT-qPCR of PP_2643 (pcaY_PP) |
| qPP_McpP_F | CAAGGCCATAGACACCAGCA | RT-qPCR of PP_2861 (mcpP) |
| qPP_McpP_R | CGTTCAATGCGGTGAGGTTG | RT-qPCR of PP_2861 (mcpP) |
| qPP_4521_F | GAAACCATCAAGCAGGGCAA | RT-qPCR of PP_4521 (aer3) |
| qPP_4521_R | TGATCCGGCCTTGTTCGTAT | RT-qPCR of PP_4521 (aer3) |
| qPP_McpS_F | ATCCAGTCGATGAACCAGCA | RT-qPCR of PP_4658 (mcpS) |
| qPP_McpS_R | TTTGCTCTGACACATCACGC | RT-qPCR of PP_4658 (mcpS) |
| qPP_McpQ_F | CCGTGATGTACTGGGTAGCA | RT-qPCR of PP_5020 (mcpQ) |
| qPP_McpQ_R | CGTGCTGGTACTGGTTGATG | RT-qPCR of PP_5020 (mcpQ) |
| qPP_0584_F | ATTTCAAGCAGCTGGGAAGC | RT-qPCR of PP_0584 |
| qPP_0584_R | CATGTAGTGCACGCCATTGA | RT-qPCR of PP_0584 |
| qPP_0779_F | AGCAAGAAATCCGGCAACTG | RT-qPCR of PP_0779 |
| qPP_0779_R | TTGACCATCTCGATCCGTCC | RT-qPCR of PP_0779 |
| qPP_1819_F | ACAACCTTGATGTGCTGCAG | RT-qPCR of PP_1819 |
| qPP_1819_R | TTGCTCAAGAAACTCGCCAC | RT-qPCR of PP_1819 |
| qPP_1940_F | CGTGTTTGAGCCGTTCTTCA | RT-qPCR of PP_1940 |
| qPP_1940_R | GAACCGACCCGCTTATTGTC | RT-qPCR of PP_1940 |
| qPP_2310_F | CGACAACTAGCCGAGGACAG | RT-qPCR of PP_2310 |
| qPP_2310_R | GCTTCGATGGCAGCGTTAAG | RT-qPCR of PP_2310 |
| qPP_2823_F | CCAGGCATAACATCGACAGC | RT-qPCR of PP_2823 |
| qPP_2823_R | CTTCCAGTTGTTCCATGGCC | RT-qPCR of PP_2823 |
| qPP_3414_F | AGCAGATTTCCCAGGAGCTT | RT-qPCR of PP_3414 |
| qPP_3414_R | CCCGAATGGTCAGCACAATC | RT-qPCR of PP_3414 |
| qPP_3557_F | AATTGGGCAGCAACGAAACC | RT-qPCR of PP_3557 |
| qPP_3557_R | GTTGGCCATGTCATGTTCGG | RT-qPCR of PP_3557 |
| qPP_3950_F | TACCTGTTCATGGCGCAATC | RT-qPCR of PP_3950 |
| qPP_3950_R | ACCGTTCTTGAGGTTTTCGC | RT-qPCR of PP_3950 |
| qPP_4888_F | CATTCAGGCGCACATTACCG | RT-qPCR of PP_4888 |
| qPP_4888_R | AACAACCCTTCACTCGCCTT | RT-qPCR of PP_4888 |
| qPP_4989_F | CATCAACGGCATGGACAACA | RT-qPCR of PP_4989 |
| qPP_4989_R | GATGTCGCCGATTTCCTGTG | RT-qPCR of PP_4989 |
| qPP_5021_F | TAGGCCTGATCAACGACCTG | RT-qPCR of PP_5021 |
| qPP_5021_R | TGGTCGAGGATGTTGCTGAT | RT-qPCR of PP_5021 |
| qPP_0387_F | AGGAAATCAACCGTCGCATG | RT-qPCR of PP_0387 (rpoD) |
| qPP_0387_R | GGTTGGTGTACTTCTTGGCG | RT-qPCR of PP_0387 (rpoD) |
| qPP_0013_F | CCGCGAAGAGTACAACATCG | RT-qPCR of PP_0013 (gyrB) |
| qPP_0013_R | ACGGAAGAAGAAGGTCAGCA | RT-qPCR of PP_0013 (gyrB) |