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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Neurobiol Dis. 2018 Nov 15;124:152–162. doi: 10.1016/j.nbd.2018.11.011

Figure 2– Microglial Progranulin Reduction with LysM-Cre Does Not Produce Lipofuscinosis, Gliosis, or Social Dominance Deficits.

Figure 2–

Microglial progranulin reduction with LysM-Cre in 12–13 month-old Grnfl/fl mice failed to produce the lipofuscinosis (A, RM ANOVA effect of LysM-Cre, p = 7569, n = 4–5 mice per group), astrogliosis (B, RM ANOVA effect of LysM-Cre, p = 0.6618, n = 5–10 mice per group), or microgliosis (C, RM ANOVA effect of LysM-Cre, p = 0.6871, n = 4–7 mice per group) that are typically observed in global Grn–/– mice. At age 13–14 months, microglial progranulin reduction also failed to produce the social dominance deficits (D, Mann-Whitney test, p = 0.1931, n = 18 mice per genotype) observed in global progranulin-insufficient mice and neuronal progranulin-insufficient mice. Microglial progranulin reduction also failed to significantly impair the amygdala response to a novel, social environment (E), though there was a clear trend (RM ANOVA effect of LysM-Cre, p = 0.0627, n = 14–20 mice per genotype) for less amygdala activation in LysM-Cre microglial progranulin-insufficient mice. Scale bars for lipofuscin, GFAP, and CD68 images represent 50 µm, the scale bar for c-Fos images represents 20 µm. VPM/VPL = ventroposteromedial/ventroposterolateral thalamus, BLA = basolateral amygdala, CeA = central amygdala, MeA = medial amygdala. † = p < 0.1.