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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: J Immunol. 2019 Jan 14;202(4):1153–1162. doi: 10.4049/jimmunol.1801323

FIGURE 2. ML-RR-cGAMP-D induces antibody responses against all four DENV NS1 serotypes and skews immunity toward the Th1 phenotype in WTB6 mice.

FIGURE 2.

Mice were immunized twice with 20 μg of NS1 from each DENV serotype in combination with ML-RR-cGAMP-D or MPLA formulated in AddaVax. Total IgG titers against NS1 from DENV1–4 were assessed by direct ELISA 1 week after the second immunization in WTB6 (A) and Ifnar−/− (B) mice. Anti-NS1 IgG2c responses in WTB6 (C) and Ifnar−/− (D) mice were used as surrogate markers of Th1 responses. Titers are shown as area under the curve (AUC) based on serially diluted serum samples. Data are representative of 2 independent experiments (mean ± SEM; n=4; * p<0.05, ** p<0.01).