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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Aug 6;143(4):1549–1559.e6. doi: 10.1016/j.jaci.2018.07.022

Figure 3: Suppression of V617F activation by secondary mutations restores cytokine sensitivity.

Figure 3:

A: STAT5 reporter assay in the presence of transfected EPOR-HA. B: IFNγ/STAT1 reporter. A and B as described for Figure 2. C: quantitative PCR (qPCR) of IFNγ-induced interferon regulatory factor 1 (IRF1). Averages and standard deviations from two biological replicates each done in technical triplicates in qPCR are shown. Mutations are color-coded by type as in Figure 2.