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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Aug 6;143(4):1549–1559.e6. doi: 10.1016/j.jaci.2018.07.022

Figure 5: Suppressing mutations localize correctly to the membrane, but differentially affect dimerization of JAK2-EPOR and kinase activity of recombinant JAK2 JH2-JH1.

Figure 5:

A: Representative confocal microscopy micrographs of fixed γ2A cells expressing the indicated JAK2-YFP mutations. B: Analysis of basal JAK2-EPOR dimerization. Normalized apparent FRET efficiency calculated from manually segmented cell membranes as detailed in Materials and Methods. Number of individual cells analyzed for each condition is indicated. Significance assessed by Student’s t test (unpaired). n.s. = not significant; *p < 0.05. C: Immunoblot analysis of whole-cell lysate from γ2A cells transiently transfected with the JAK2-HA constructs and EPOR-HA as shown. D: Kinase assay with purified recombinant JAK2 JH2-JH1. Shown are averages and standard deviation from triplicate measurements.