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. 2019 Feb 6;39(6):1030–1043. doi: 10.1523/JNEUROSCI.2143-18.2018

Figure 3.

Figure 3.

CRFCeA neurons target the dlBST. A, Configuration of dual viral injections into the dlBST and CeA of Crh-Cre rats for anterograde tracing. B, A dense plexus of mCherry+ CRFCeA fibers was detected in the dlBST in the vicinity of eYFP+ CRFdlBST neurons. Scale bar, 200 μm. LV, Lateral ventricle; Str, striatum; ac, anterior commissure. C, Configuration of dual viral injections into the dlBST and CeA of Crh-Cre rats for retrograde tracing. D, Many mCherry+ CRF neurons in the CeA were retrogradedly labeled with CAV2-packaged ZsGreen. Scale bar, 200 μm. Boxed region is enlarged in inset. Scale bar, 50 μm. E, Quantification of ZsGreen expressed in CeA cell bodies revealed that ∼46.1% (total of 1465 neurons counted from 3 to 6 BST sections from 5 rats) of CRFCeA (mCh+) neurons project to the dlBST. F, CRFCeA inputs cluster around PKCδ neurons in the oval nucleus. Scale bar, 200 μm. G and H, CRFdlBST neurons are distinct from PKCδ neurons (G) and from cells expressing proenkephalin (H). Scale bars: G, 200 μm; H, 50 μm. mCh (mCherry), LV (lateral ventricle), pEnk (proenkephalin).