Figure 6.
Extended amygdala CRF network for eliciting anxiety. A, AAV encoding Cre-dependent KORD was injected into the dlBST, and AAV encoding Cre-dependent hM3Dq was injected into the ipsilateral CeA. B, Example of antibody-amplified mCitrine fluorescence in CRFdlBST neurons and native mCherry in CRFCeA neurons. Scale bars, 200 μm. C, Top, Experimental protocol. Bottom, Inhibition of CRFdlBST neurons with SalB prevented CNO-induced decreases in the percentage of time in the open arms of the EPM and reductions of open arm entries on the EPM without affecting closed arm entries. SalB also prevented CNO-induced decreases in time spent in the center of the OF. D, In the OF, CNO reduced the time spent in the center and distance traveled in the center, but coadministration of SalB prevented this effect without affecting the total distance traveled. Data are mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001. Veh (vehicle), LV (lateral ventricle), Str (striatum), ac (anterior commissure), BLA (basolateral amygdala).