Anterior insular cortex inactivation prevents context-induced relapse to alcohol seeking in Context B, the punishment context following prolonged abstinence. Outline of the experimental timeline (A). Number of active lever presses (left) and latency to first lever press (right) in the punishment context following prolonged abstinence (B). Approximate cannula placements (mm from bregma) of the injector tips for the AI (C). SAL, saline, M+B, muscimol + baclofen. Data are presented as mean ± SEM. n = 8–9/group. Scale bar, 1000 μm. *p < 0.05.