Figure 6.
TAC activates human TRPA1 channels. A, Averaged ± SEM fura-2 fluorescence ratio of HEK292 cells transfected with human TRPA1 (blue trace), rat TRPV1 (black trace), or mouse TRPM3 (green trace) during applications of TAC (30 μm) and their canonical agonists capsaicin (100 nm), AITC (50 μm), and pregnenolone sulfate (PS, 50 μm). B, Bar histogram summarizing the effect of TAC or the canonical agonists, capsaicin, AITC, or PS, on fura-2 fluorescence ratio. Individual records have been baseline-subtracted. TAC produced a significant elevation in [Ca2+]i in TRPA1-transfected cells compared with TRPV1- or TRPM3-transfected cells (***p < 0.001, one-way ANOVA followed by Bonferroni's post hoc test). ***p < 0.001.