Synaptic inputs from the MLR to stop cells. A, Response of a stop cell to a pair of electrical shocks delivered to the MLR at different frequencies (20, 40, 60, and 80 Hz). The black traces represent average cellular responses from one of four recorded stop cells (n = 10 sweeps; gray traces). B, Double electrical shocks were delivered to the MLR at 25 Hz while a stop cell was recorded intracellularly. To reduce the likelihood of polysynaptic transmission, a high-divalent cation Ringer's solution was applied in the recording chamber (right, blue box) (El Manira et al., 1997; Brocard and Dubuc, 2003). Black traces represent average cellular responses from one of three recorded stop cells (n = 10 sweeps; gray traces).