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. 2018 Dec;7(4):165–172. doi: 10.22099/mbrc.2018.30979.1354

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Restriction digestion of confirmed colony PCR positive pZ57R/T mce-whole clones: lane M: 1kb DNA ladder (#SM0313, Fermentas), lanes 1, 2 and 3: Positive clones of PTZ57R/T mce-whole plasmid digested with BamHI and HindIII enzymes; Lanes 5, 6 and 7: Plasmid isolated of positive PTZ57R/T mce-whole clones. (B) Restriction Digestion of confirmed colony PCR positive pET28a mce-whole clones; lane M: 1kb DNA ladder (#SM0313, Fermentas),lanes 2 and 4: Positive clones of pET28a mce-whole plasmid.