Figure 4.

Remodeling factors are increased in the lungs of naive CC16 (club cell secretory protein 16) knockout mice. (A) Representative pentachrome-stained lung sections from wild-type (WT) and CC16^−/− naive mice. Scale bars: upper panels, 50 μm; lower panels, 100 μm. (B) Quantitative analysis of smooth muscle thickness was measured on pictures taken at ×10 magnification using ImageJ software as described in the Methods section. n = 8 and 7 WT and CC16^−/−, respectively. (C–G) Quantitative RT-PCR analysis for Pro-Col1A1 (C), Pro-Col3A (D), α-sma (E), Tgf-β (F), and Pdgf (G) gene expression in lung tissue from naive WT and CC16^−/− mice. Graphed gene expression data are the mean ±  SD, **P < 0.01 by Student’s t test. n = 12 and 16 WT and CC16^−/−, respectively. Ave = average; FC = fold over control; PDGF = platelet-derived growth factor; Pro-Col = procollagen; SM = smooth muscle; SMA = smooth muscle actin; TGF = transforming growth factor.