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. 2019 Feb 5;93(4):e01446-18. doi: 10.1128/JVI.01446-18

FIG 9.

FIG 9

CD4 binding to HIV-1 Env and the inhibitory effect of BMS-626529 and bNAbs. (a) CD4 binding to HIV-1 JRFL Env trimer was measured by Env binding ELISA. Competitors, including CD4-IgG2, sCD4, BMS-626529, and bNAbs, were added at 25 nM final concentration. Samples without CD4-IgG2 coating or JRFL Env trimer were controls. Binding data of sample with competitor were normalized to the no-competitor control. (b) Binding of CD4 to HIV-1 JRFL Env trimer with treatment of BMS-626529 or NIH45-46G54W or the two in combination at the indicated concentrations. The binding data of sample with competitor(s) were normalized to the no-competitor control. The data represent results from three independent assays performed with duplicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (multiple t tests). (c) Highest single agent (HSA) synergy and antagonism matrix of BMS-626529 and bNAb NIH45-46G54W at indicated concentrations in blocking CD4 binding to HIV-1 JRFL gp140. The data represent results of two independent assays.