FIG 8.

Autophagy facilitates IAV RNA synthesis by regulating HSP90AA1 expression. (A) HEK 293T cells were transfected with siATG5 for 36 h and then transfected with the indicated plasmids. Twenty-four hours later, cell lysates were harvested for Western blot analysis. (B) siHSP90AA1-transfected HEK 293T cells were transfected with indicated plasmids for 24 h, and lysates were subjected to Western blot analysis. (C) Lysates of PCDNA3.1-PB2-transfected HEK 293T cells were prepared and immunoprecipitated with the anti-PB2 antibody or control IgG, and then cells were subjected to Western blot analysis. Results are representative of three independent experiments.