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. 2019 Feb 5;93(4):e01306-18. doi: 10.1128/JVI.01306-18

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FIG 5 — Mapping of RHA domains that interacted with viral NS proteins. (A) Schematic representation of full-length RHA-FLAG and three truncated forms of RHA. 293T cells were transfected with plasmids encoding different regions of RHA and DENV2 NS1-HA (B), NS2B3-HA (C), or NS4B-HA (D). The whole-cell extracts were prepared at 48 h posttransfection and immunoprecipitated with FLAG antibody. Western blot was probed with FLAG or HA antibody. RHA-FLAG, full-length RHA tagged with FLAG at C terminus; RHAN-FLAG, N-terminal fragment of RHA tagged with FLAG at C terminus; RHAM-FLAG, intermediate fragment of RHA tagged with FLAG at C terminus; RHAC-FLAG, C-terminal fragment of RHA tagged with FLAG at C terminus. Data are representative of three independent experiments. Asterisks indicate nonspecific bands.