FIG 6.

Expression of RHA^RSC or RHA-K417R rescued viral replication levels. (A and B) Control cells or RHA^RSC- or RHA-K417R (K417R)-expressing cells were transfected with siNC or siRHA1. At 48 h posttransfection, cells were infected by DENV2 at an MOI of 3. Cells and supernatants were collected at 24 h p.i. for Western blotting (A) and plaque assay (B). Western blot was probed with antibodies against RHA, FLAG, NS3, and GAPDH. (C) Co-IP assay. The RHA^RSC- or RHA-K417R-expressing cells were infected by DENV2 at an MOI of 3. At 24 h p.i., cells were harvested for co-IP assay using anti-FLAG antibody. Protein complexes were separated by SDS-PAGE and detected by Western blotting with antibodies against the indicated proteins. Data are representative of three independent experiments. ***, P < 0.001 by unpaired, two-tailed Student's t test.