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. 2019 Feb 5;93(4):e01306-18. doi: 10.1128/JVI.01306-18

TABLE 1.

Sequences of primers used in PCR

Gene name Sequence (5′–3′)
RHA-FLAG 5F, ATGGGTGACGTTAAAAATTTTCTGTATGC
3R, CGCGGATCCTTACTTATCGTCGTCATCCTTGTAATCACCACCACCATAGCCGCCACCTCCTCTTCCCTG
RHAN-FLAG 5F, CCGGAATTCTATGGGTGACGTTAAAAATTTTCTGTATGC
3R, CGCGGATCCCTACTTATCGTCGTCATCCTTGTAATCACCACCACCTGTCTCTCCTTCCTTCTTCTTTGTAAGTCC
RHAM-FLAG 5F, CCGGAATTCATGGTGGAGCCTTACAAAGTAAACC
3R, CGCGGATCCCTACTTATCGTCGTCATCCTTGTAATCACCACCACCATCAAGCTCTCTAAGAGTGTGTT
RHAC-FLAG 5F, CCGGAATTCATGGCATTAGATGCCAATGATGAGTTGACTCC
3R, CGCGGATCCTTACTTATCGTCGTCATCCTTGTAATCACCACCACCATAGCCGCCACCTCCTCTTCCCTG
RHA(RSC) mutation 5F, TGCACTTATCCATAAGTCGTCTGTCAATTGCCCTTTTAGT
3R, ACTAAAAGGGCAATTGACAGACGACTTATGGATAAGTGCA
Lentivirus-RHA 5F, ATTTGCGGCCGCATGGGTGACGTTAAAAATTTTCTGT
    (RSC)-FLAG 3R, CGCGGATCCTTACTTATCGTCGTCATCCTTGTAATCACCACCACCATAGCCGCCACCTCCTCTTCCCTG
RHA(K417R) mutation 5F, CTGGATGTGGGAGAACCACACAGG
3R, CTGGATGTGGGAAAACCACACAGG
Lentivirus-RHA 5F, ATTTGCGGCCGCATGGGTGACGTTAAAAATTTTCTGT
    (K417R)-FLAG 3R, CGCGGATCCTTACTTATCGTCGTCATCCTTGTAATCACCACCACCATAGCCGCCACCTCCTCTTCCCTG
RHA siRNA resistance mutation 5F, CTGATGGGCAGATTGTATTAGTCGACGATTGGATCAAACTGCAAATATCTCAT
3R, ATGAGATATTTGCAGTTTGATCCAATCGTCGACTAATACAATCTGCCCATCAG
NS1-HA 5F, CGCGGATCCATGGATAGTGGTTGCGTTGT
3R, CCGCTCGAGCTACTTATCGTCGTCATCCTTGTAATCACCACCACCAGCTGTGACCAAGGAGTT
NS2A-HA 5F, CGCGGATCCATGGGACATGGGCAGATTGACAACTT
3R, CCCAAGCTTCTAAGCGTAATCTGGAACATCGTATGGGTAACCACCACCCCTTTTCTTGTTGGTTCTTGAAAG
NS2B3-HA 5F, CGCGGATCCATGAATAACCAACGAAAAAAGGCG
3R, CCCAAGCTTCTAAGCGTAATCTGGAACATCGTATGGGTAACCACCACCCGCCATCACTGTTGGAATCAG
NS4A-HA 5F, CCCGGATCCATGTCCCTGACCCTGAACCTAATCACAGAA
3R, AAGCTTTAGAGCGTAATCTGGAACATCGTATGGGTAACCACCACCTCTTTTCTGAGCTTCTCTGGT
NS4B-HA 5F, CGCGGATCCATGAACGAGATGGGTTTCCTGGAA
3R, CCCAAGCTTCTAAGCGTAATCTGGAACATCGTATGGGTAACACCACCCCTTCTCGTGTTGGTTGTGTTCTT
NS5-HA 5F, CCCAAGCTTATGGGAACTGGCAACATAGGAG
3R, GCTCTAGACTAAGCGTAATCTGGAACATCGTATGGGTAACCACCACCCCACAAAACTCCTGCCTCT
IFNAR1 sgRNA 5F, CACCGGATCTAATGTTAAAGACTGG
3R, AAACCCAGTCTTTAACATTAGATCC