FIG 3.

Cytokine and ISG expression levels in WT and STING N153S mouse lung homogenates prior to and during infection with γHV68. (A to E) WT and STING N153S mice were inoculated intranasally with 2 × 10⁵ PFU of γHV68. Animals were euthanized at day 8 or 14 after infection; mock-infected animals were euthanized on day 0. Cytokine and chemokine levels were assessed by a Luminex assay of lung homogenates. Data represent the means ± standard errors of the means of results for 7 to 9 mice per group at each time point, pooled from 2 independent experiments. (F to O) STING N153S and WT littermate control mice were inoculated intranasally with 2 × 10⁵ PFU of γHV68. Animals were euthanized at day 4, 8, or 14 after infection; mock-infected animals were euthanized on day 0. ISG (F to J) and cytokine (K to O) gene expression levels in lung homogenates were assessed by qRT-PCR and are reported as the fold change relative to expression levels for the WT. Results from 6 mice per group at each time point were pooled from 2 independent experiments. All data represent the means ± standard errors of the means from 2 independent experiments. Results were analyzed by the Mann-Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.