TABLE 1.

Cytokine levels in WT and STING N153S lung homogenates prior to and during infection with γHV68^a

Cytokineb	LODc (pg/g)	Level (pg/g of tissue)
		Mock infection				Day 8 after infection				Day 14 after infection
		WT		STING N153S		WT		STING N153S		WT		STING N153S
		Mean	SD	Mean	SD	Mean	SD	Mean	SD	Mean	SD	Mean	SD
IL-1α	2.25	2,950	1,873	4,741	2,892	892	353	5,757	7,886	858	249	2,343	1,304
IL-1β	1.85	1,937	1,339	2,508	1,225	417	244	2,459	3,319	592	502	1,079	675
IL-2	0.88	1,858	2,040	1,472	675	398	160	337	123	325	190	159	328
IL-3	0.45	46	190	67	142	9	16	16	28	111	201	11	22
IL-4	0.32	123	304	137	245	160	92	115	97	45	93	11	5
IL-5	0.59	960	778	812	297	262	142	279	141	287	176	155	94
IL-6	0.71	699	626	1,865	1,860	325	231	3,608	3,249	212	155	12,467	10,306
IL-9	2.36	1,958	2,263	1,016	1,839	617	255	462	394	398	900	62	32
IL-10	3.57	1,789	1,541	3,011	1,521	3,438	2,240	2,995	1,423	1,394	1,057	1,943	855
IL-12(p40)	8.81	7,539	5,501	52,699	24,545	20,226	16,461	90,693	93,701	11,844	7,296	30,459	19,296
IL-12(p70)	4.28	1,711	1,739	1,613	1,096	1,106	456	1,260	681	766	712	783	509
IL-13	10.96	10,266	10,463	8,335	6,455	672	713	652	666	3,939	6,600	1,220	1,743
IL-17A	0.49	450	368	281	135	93	26	158	120	140	208	52	62
Eotaxin	22.92	304,216	642,378	703,751	1,263,328	931,207	834,322	499,161	843,408	34,380	13,778	10,237	3,474
G-CSF	3.97	1,211	2,381	3,390	2,737	281	312	11,874	16,821	698	1,212	85,054	86,806
GM-CSF	21.6	2,281	1,172	2,370	1,111	22	0	843	1,030	1,343	1,436	1,867	1,202
IFN-γ	0.92	2,809	1,887	1,921	948	513	329	302	226	878	280	554	541
KC	1.15	12,785	19,214	40,670	34,909	5,162	3,204	28,292	12,174	5,083	2,533	32,282	26,956
MCP-1	43.09	36,610	43,423	50,221	25,564	42,790	31,808	33,136	48,194	19,537	11,692	256	132
MIP-1α	0.43	3,756	2,050	9,068	5,282	12,726	10,362	14,477	8,434	8,418	5,705	33,277	13,085
MIP-1β	2.61	6,274	7,863	5,688	6,137	5,252	2,057	3,028	424	2,001	525	1,699	987
RANTES	1.62	82,621	67,099	164,089	120,460	530,764	601,268	24,005	56,366	142,988	69,619	794	912
TNF-α	5.05	23,864	14,393	21,234	6,908	1,934	608	2,036	678	7,094	10,641	8,753	7,290

^aWT and STING N153S mice were inoculated intranasally with 2 × 10⁵ PFU of γHV68. Animals were euthanized at day 8 or 14 after infection; mock-infected animals were euthanized on day 0. Cytokine and chemokine levels were assessed by Luminex assays of lung homogenates. Results for selected cytokines are displayed graphically in Fig. 3. Data represent results for 7 to 9 mice per group at each time point, pooled from 2 independent experiments.

^bMCP-1, monocyte chemoattractant protein 1.

^cLOD, limit of detection.