FIG 11.

VLoop sequences in both CHIKV and SFV nsP2 proteins play critical roles in protein function in RPB1 degradation. (A) Schematic presentation of VEEV replicons encoding different forms of SINV, CHIKV, and SFV nsP2 fused with GFP. (B) NIH 3T3 cells were infected with indicated packaged replicons at an MOI of 20 infectious units/cell. At 8 h p.i., cells were harvested and cell lysates were analyzed by Western blotting using RPB1-, GFP-, and tubulin-specific Abs. Membranes were scanned on an Odyssey imager (LI-COR) and processed using the manufacturer’s software. The experiment was repeated twice, with reproducible results.