FIG 3.

The QQA mutation in CHIKV nsP2 VLoop impairs P12 processing. (A) Schematic presentation of recombinant CHIKV genomes encoding either mutated VLoop or mutated nsP2/3 cleavage site. (B) NIH 3T3 cells were infected with the indicated viruses at an MOI of 20 PFU/cell and harvested at 8 h p.i. The presence of individual nsPs and incompletely cleaved polyproteins in cell lysates was analyzed by Western blotting using antibodies specific to CHIKV nsP1, nsP2, and nsP3 proteins. Membranes were analyzed on an Odyssey imager (LI-COR).