FIG 8.

Mutations in VLoop do not affect nuclear translocation of CHIKV nsP2. NIH 3T3 cells were infected with indicated recombinant viruses and at 6 h p.i., fixed with 4% PFA, permeabilized, and stained with pan-anti-nsP2 MAb, anti-mouse secondary antibodies labeled with Alexa Fluor 555 (Thermo Fisher) and Hoechst nuclear staining. The image stacks of 6 optical sections were acquired on a Zeiss LSM700 confocal microscope with a 63 × 1.4 numerical aperture (NA) PlanApochromat oil objective. The multiple intensity projection images were assembled using Imaris software (Bitplane AG).