FIG 9.

The designed CHIKV mutants do not induce transcriptional shutoff in the infected cells and inefficiently downregulate translation of cellular mRNAs. (A) NIH 3T3 cells were infected with indicated viruses at an MOI of 20 PFU/cell. RNAs were metabolically labeled with [³H]uridine in the absence of ActD between 4 and 8 h p.i. Labeled RNAs were analyzed by agarose gel electrophoresis under denaturing conditions as described in Materials and Methods. (B) NIH 3T3 cells were infected at an MOI of 20 PFU/cell, and at 6 h p.i., proteins were metabolically labeled for 30 min with [³⁵S]methionine and analyzed by SDS-PAGE as described in Materials and Methods.