FIG 1.

DHX9 helicase interacts with CHIKV nsPs in infected cells. Total lysates prepared from uninfected HeLa cells (NI) or from cells infected with CHIKV for 8 h were immunoprecipitated with anti-DHX9 (A) or anti-nsP3 serum (B). Samples of lysates (Input) and immunoprecipitates (IP) were separated by SDS-PAGE and probed with antisera against DHX9, CHIKV nsP1, nsP2, nsP3, or GAPDH MAbs as indicated. (C) HEK293T cells were cotransfected to express HA-DHX9, together with GFP or GFP-fused nsPs, as indicated. Cell lysates were prepared 36 h after transfection, precipitated with anti-HA agarose affinity beads, and separated by SDS-PAGE. Lysates and precipitated complexes were probed with MAbs against GFP, HA, or GAPDH. For each panel, the molecular masses are indicated.