FIG 3.

CHIKV nsP3 binds DHX9 through its C-terminal HVD. (A) Schematic representation of GFP-nsP3 and its truncated derivatives. (B) HEK293T cells transfected to coexpress HA-DHX9, together with proteins depicted in panel A or with GFP, were stained with anti-DHX9 serum. Nuclei were stained with DAPI, and the cells were imaged by confocal microscopy. Scale bar, 5 μm. (C) Total cell extracts were prepared for each condition and precipitated with anti-HA agarose affinity beads. Proteins and bound complexes were visualized using antibodies against GFP, HA, or GAPDH, as indicated. Molecular masses are indicated.