FIG 4.

Consequences of CHIKV infection on DHX9 levels and its subcellular localization. (A) HeLa cells infected for 8 h with the CHIKV-377-mCherry virus (MOI = 0.5) were fixed and stained with antibodies against DHX9 and dsRNA. Uninfected cells (NI) are shown as a control. Scale bars, 10 μm. (B) The intensity of DHX9 nuclear staining in nsP3⁺ or nsP3^– cells from CHIKV infection condition or in uninfected (NI) culture was determined using ImageJ software. Mean values were compared using a Student t test. ***, P < 0.001. (C) Total lysate of uninfected cells or that from cells infected with CHIKV for 16 h in the absence or presence of ribavirin were probed with anti-DHX9, anti-nsP3, and anti-capsid antibodies; anti-tubulin MAb was used as a loading control. (D) Infected cells maintained in medium alone (CHIKV) or culture in the presence of 10 μM ALLN (CHIKV+ALLN) were collected at different times postinfection and analyzed by immunoblotting with antibodies specific to DHX9, nsP3, and GAPDH.