FIG 5.

DHX9 is recruited to nsP3/G3BP complexes formed in the cytoplasm of CHIKV-infected cells. HeLa cells transfected to express the G3BP-GFP protein were left uninfected (NI) or were infected with the CHIKV-377-mCherry virus for 8 h. Cells were stained with anti-dsRNA antibodies (A) or anti-DHX9 serum (B) and processed for confocal imaging. Arrows indicate cytoplasmic foci with colocalized signals. For each panel, the lower lane shows an enlargement of the boxed zone. (C) Protein extracts prepared from uninfected cells or cells infected with the CHIKV-377-mCherry reporter virus for the indicated time were processed for immunoblot analysis of DHX9, nsP3, and G3PB expression (Input). Proteins complexed with nsP3 were immunoprecipitated (IP) with anti-mCherry MAbs and revealed by specific antibodies, as indicated.