TABLE 1.

Primer sequences

Primer	Sequence (5′–3′)	Positions (nt)
nsP1-Fw	AGATCTCGAGCTCAAGCTTCGATGGATCCTGTGTACGTG	77–94
nsP1-Rev	TTAACCGTCGACTGCAGATCCTGCGCCCGCTCTGTC	1667–1686
nsP2-Fw	TCCGGACTCAGATCTCGAGCTATAATAGAGACTCCGAGAGGA	1685–1705
nsp2-Rev	GGATCCCGGGCCCCGCGGTACCACATCCTGCTCGGGTGAC	4058–4076
nsP3-Fw	TCCGGACTCAGATCTCGAGCTGCACCGTCGTACCGGGTA	4075–4093
nsP3-Rev	GGATCCCGGGCCCGCGGTACCCACCTGCCCTGTCTAG	5648–5665
nsP4-Fw	TCCGGACTCAGATCTCGAGCTTATATATTCTCGTCGGAC	5666–5683
nsp4-Rev	GGATCCCGGGCCCGCGTACCCTATTAGGACCGCCGTA	7484–7507
Macro-Fw	CAAGCTTCGAATTGTGCACCGTCGTACCGGGTAAAA	4083–4096
Macro-Rev	AGATCCGGTGGATCCTCACAGCTCTACTTGGGTCCGCAT	4547–4570
AUD-Fw	CAAGCTTCGAATTCTCTGGATAGCACATCTCCTAG	4564–4585
AUD-Rev	AGATCCGGAGGATCCTCAATATTCCCTTGGACTTACG	5029–5047
HVD-Fw	CAAGCTTCGAATTCTAGTCTTCCCAGGAGTCTGC	5051–5067
HVD-Rev	AGATCCGGTGGATCCTCAACCTGCCCTGTCTAGTCTTAA	5660–5680
CHIKV-F	GGCAGTGGTCCCAGATAATTCAAG	3170–3193
CHIKV-R	GCTGTCTAGATCCACCCCATACATG	3229–3253
CHIKV(–)	GGCAGTATCGTGAATTCGATGCCGCTGTACCGTCCCCATTCC	6220–6239
CHIKV(–)F	GGCAGTATCGTGAATTCGATGC	6354–6364
CHIKV(–)R	ACTGCTGAGTCCAAAGTGGG	6311–6330