FIG 9.

Differential distribution of viral genomes is independent of antiviral signaling. (A and B) RT-qPCR for IFNB (A) and IFIT1 (B) mRNAs relative to GAPDH (n = 3). **, P < 0.005 by one-way ANOVA followed by a Bonferroni post hoc test. (C to E) CRISPR control A549 (C), MAVS KO (D), and IFNAR KO (E) cell lines were infected with SeV-HD for 24 h and then subjected to positive-sense viral RNA-FISH. Wide-field images were captured with a 63× objective. Deconvolved, extended focus is shown. Images are representative of results from three independent experiments. Bar = 50 μm.