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Subgenomic vRNAs in virus particles. Released viruses from MDCK cells, infected at an MOI of 10 (from the experiment shown in Fig. 4B), were investigated for the presence of subgenomic vRNAs on S1 to S8 by segment-specific RT-PCR at 12 hpi. FL and DI vRNAs appear in the top and bottom parts of the gel, respectively.
