TABLE 2.

Primers for qPCR (related to real-time RT-qPCR)

Target	RNA type(s)	Primer name	Sequence (5′→3′)
Introduced tag sequence	vRNA	vRNA tagRealtime for	ATTTAGGTGACACTATAGAAGCG
	cRNA	cRNA tagRealtime rev	GCTAGCTTCAGCTAGGCATC
	mRNA	mRNA tagRealtime rev	GTAAAACGACGGCCAGT
Segment 5	vRNA	Seg 5 Realtime rev	CGCACTGGGATGTTCTTC
	cRNA and mRNA	Seg 5 Realtime for	GGAAAGTGCAAGACCAGAAGAT
Segment 7	vRNA	Seg 7 Realtime rev	TGAGCGTGAACACAAATCCTAAAA
	cRNA and mRNA	Seg 7 Realtime for	CATTGGGATCTTGCACTTGACATT
Segment 8	vRNA	Seg 8 Realtime rev	CACTTTCTGCTTGGGTATGA
	cRNA and mRNA	Seg 8 Realtime for	GGCGGGAACAATTAGGTCAGA